Molecular hydrogen suppresses FcεRI-mediated signal transduction and prevents degranulation of mast cells
by Kinji Ohno, Mikako Ito, Masatoshi Ichihara, Masafumi Ito, Akio Masuda, Tomohiro Itoh, Yasunori Fujita, Yoshinori Nozawa, Toshio Kojima
Abstract:
Molecular hydrogen ameliorates oxidative stress-associated diseases in animal models. We found that oral intake of hydrogen-rich water abolishes an immediate-type allergic reaction in mice. Using rat RBL-2H3 mast cells, we demonstrated that hydrogen attenuates phosphorylation of the FcεRI-associated Lyn and its downstream signal transduction, which subsequently inhibits the NADPH oxidase activity and reduces the generation of hydrogen peroxide. We also found that inhibition of NADPH oxidase attenuates phosphorylation of Lyn in mast cells, indicating the presence of a feed-forward loop that potentiates the allergic responses. Hydrogen accordingly inhibits all tested signaling molecule(s) in the loop. Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. Effects of hydrogen in other diseases are possibly mediated by modulation of yet unidentified signaling pathways. Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide.
Read more:
https://doi.org/10.1016/j.bbrc.2009.09.047
Related Articles:
Molecular hydrogen has an anti-inflammatory and cardioprotective effect, which is associated with its antioxidant properties. Erythrocytes are subjected to oxidative stress in pathologies of the cardiovascular system, which is the cause of a violation of the gas transport function of…
Neutrophil extracellular traps (NETs) contribute to inflammatory pathogenesis in numerous conditions, including infectious and cardiovascular diseases, and have attracted attention as potential therapeutic targets. H2 acts as an antioxidant and has been clinically and experimentally proven to ameliorate inflammation. This…
Objective To investigate the therapeutic effect and mechanism of hydrogen (H2) on collagen-induced arthritis (CIA) in mice. Methods DBA/1 mice were randomly divided into normal control group, CIA group and CIA mice treated with H2 group (H2 treated group), with…
Aims: Molecular hydrogen (H2) has attracted growing interest because of its implications in various diseases. However, the molecular mechanisms underlying the remarkable effect of a small amount of H2 remain elusive. No knowledge has been available on the role of…
Aim: To study the inhalation of an active form of hydrogen effect to mucosal and system immunity in a rehabilitation program for health workers. Materials and methods: The study involved patients that survived COVID-19 after therapy with inhaled hydrogen for…
Hydrogen inhalation therapy has been proven to be safe and effective in disease treatment in multiple clinical reports, but the gas flow rates used in different studies vary greatly. Since there is no upper limit for the safe concentration of…
0 Comments