Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2,2′-bipyridyl methods
by Nobuhiko Miwa, Shinya Kato, Daigo Matsuoka
Abstract:
We prepared nano-bubble hydrogen-dissolved water (nano-H water) which contained hydrogen nano-bubbles of <717-nm diameter for 54% of total bubbles. In the DMPO-spin trap electron spin resonance (ESR) method, the DMPO-OH:MnO ratio, being attributed to amounts of hydroxyl radicals (OH), was 2.78 for pure water (dissolved hydrogen [DH]≤0.01ppm, oxidation-reduction potential [ORP]=+324mV), 2.73 for tap water (0.01ppm, +286mV), 2.93 for commercially available hydrogen water (0.075ppm, +49mV), and 2.66 for manufactured hydrogen water (0.788ppm, -614mV), whereas the nano-H water (0.678ppm, -644mV) exhibited 2.05, showing the superiority of nano-H water to other types of hydrogen water in terms of OH-scavenging activity. Then, the reduction activity of nano-H water was assessed spectrophotometrically by the 2,2′-bipyridyl method. Differential absorbance at 530nm was in the order: 0.018 for pure water, 0.055 for tap water, 0.079 for nano-H water, 0.085 for commercially available hydrogen water, and 0.090 for manufactured hydrogen water, indicating a prominent reduction activity of hydrogen water and nano-H water against oxidation in ascorbate-coupled ferric ion-bipyridyl reaction. Thus, nano-H water has an improved antioxidant activity as compared to hydrogen water of similar DH-level, indicating the more marked importance of nano-bubbles rather than the concentration of hydrogen in terms of OH-scavenging. Copyright © 2015 Elsevier B.V. All rights reserved.
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https://doi.org/10.1016/j.msec.2015.03.064
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