Hydrogen gas attenuates embryonic gene expression and prevents left ventricular remodeling induced by intermittent hypoxia in cardiomyopathic hamsters
by Aiji Sakamoto, Atsuo Nomura, Kazuhiko Tanaka, Koyuha Amatani, Minoru Yoshiyama, Ryuji Kato, Sayuri Nagai, Takehiro Yamaguchi, Tetsuya Hayashi, Yasukatsu Izumi, Yoko Sen, Yoshikatsu Okada, Yoshio Ijiri, Yuki Yasuda
Abstract:
The prevalence of sleep apnea is much higher in patients with heart failure, and intermittent hypoxia (IH) relevant to sleep apnea might induce left ventricular (LV) remodeling. The aim of this study was to investigate the effect of repetitive hypoxic stress on failing heart and to evaluate the antioxidant effect of hydrogen gas. Male Syrian normal (n=22) and cardiomyopathic (CM) (n=33) hamsters were exposed to IH (5-min cycles of 5% and 21% oxygen for 8 h/day) or normoxia for 14 days. Hydrogen gas (3.05 vol/100 vol) was administered by inhalation to CM hamsters during hypoxia. After evaluating cardiac function by echocardiography, the heart was examined by light and electron microscopy, immunohistochemistry and RT-PCR. IH tended to increase the ratio of early diastolic mitral inflow to mitral annulus velocity (E/e’, 21.8 vs. 16.9), but did not affect LV ejection fraction (EF) in Syrian hamsters. In CM hamsters, however, IH increased E/e’ (29.4 vs. 21.5) and significantly decreased ejection fraction (37.2% vs. 47.2%). IH also increased cardiomyocyte cross-sectional area (672 vs. 443 μm(2)), interstitial fibrosis (29.9% vs. 9.6 %), associated with increased oxidative stress and superoxide production in LV myocardium. Furthermore, IH significantly increased c-fos and c-jun mRNA expression in CM. Hydrogen gas inhalation significantly decreased oxidative stress and embryonic gene expression, consequently preserving cardiac function in CM hamsters. IH accelerated LV remodeling of failing heart at least partly through increased oxidative stress in CM hamsters, which might account for the poor prognosis of patients with HF accompanied by sleep apnea.
Read more:
https://doi.org/10.1152/ajpheart.00228.2014
Related Articles:
We previously reported the neuroprotective potential of combined hydrogen (H2) gas ventilation therapy and therapeutic hypothermia (TH) by assessing the short-term neurological outcomes and histological findings of 5-day neonatal hypoxic-ischemic (HI) encephalopathy piglets. However, the effects of H2 gas on…
Objective: To investigate whether hydrogen-rich water exerts a protective effect against cellular injury by affecting the level of autophagy after oxygen glucose deprivation/reoxygenation (OGD/R) in a mouse hippocampal neuronal cell line (HT22 cells). Methods: HT22 cells in logarithmic growth phase…
Background: Our previous research revealed that inflammation plays an important role in the pathophysiology of cerebral ischemia. The function of the NOD-like receptor protein 3 (NLRP3) inflammasome is to activate the inflammatory process. Recent findings suggest that reactive oxygen species…
Neonatal hypoxic-ischemic encephalopathy (HIE) is a major cause of morbidity and mortality in newborns in both high- and low-income countries. The important determinants of its pathophysiology are neural cells and vascular components. In neonatal HIE, increased vascular permeability due to…
Hydrogen (H2) therapy is a therapeutic strategy using molecular H2. Due to its ability to regulate cell homeostasis, H2 therapy has exhibited marked therapeutic effects on a number of oxidative stress-associated diseases. The present study investigated the effectiveness of H2…
Background: Post-cardiac arrest (CA) brain injury is the main cause of death in patients resuscitated from CA. Previous studies demonstrated that hydrogen inhalation mitigates post-CA brain injury. However, factors affecting the efficacy of hydrogen remain unknown. In the present study,…
0 Comments