Attenuation of diffuse axonal injury by intraperitoneal injection of hydrogen via reducing oxidative stress in rats
by Peng sun, Bin-Fei Zhang, Dan-Dong Li, Jin-Ning Song, Xu-Dong Ma, Ya-Hui Zhao, Yong-Lin Zhao, Zun-Wei Liu
Abstract:
To investigate the role of oxidative stress in diffuse axonal injury (DAI) and the therapeutic action of hydrogen in DAI models. Methods Totally 118 adult male SD rats were divided into 3 groups with 39 in each: sham, DAI, and DAI + hydrogen treatment. Rat diffuse axonal injury was induced by an experimental facility, which was developed to make the rat head spin 90 degrees at the moment to cause shearing injury. Hydrogen gas of high purity was injected intraperitoneally into rats of DAI model twice per day (10mL/kg). The modified neurological severity score (mNSS), histomorphology, propidium iodide (PI) staining, SOD activity, CAT activity and MDA content in the brain were assayed at 6h, 1d, 3d and 7d after DAI. Results MDA content, PI positive cells, swelling never cells, degenerated cells, glial cells and neurological severity scores were significantly increased, while SOD and CAT activities were decreased in the brain tissues of DAI rats (P<0.01). Hydrogen treatment could reverse these changes mentioned above. Conclusion Oxidative stress is involved in DAI pathophysiological process in acute phase. The decline of antioxidant enzyme activities and production of large numbers of free radicals may lead to the deterioration of brain damage. Hydrogen gas can improve acute brain injury in DAI by improving antioxidant enzyme activities and reducing free radical damage. Reducing the level of oxidative stress is of great significance in the treatment of DAI.
Read more:
https://doi.org/10.7652/jdyxb201406007
Related Articles:
Objective: To investigate the effect of hydrogen gas on NOD-like receptor protein 3 (NLRP3) inflammasomes in the cerebral cortex of rats with traumatic brain injury (TBI). Methods: 120 adult male Sprague-Dawley (SD) rates were randomly divided into 5 groups (n…
Traumatic brain injury (TBI) has been recognized as a serious public health issue and a key contributor to disability and death, with a huge economic burden worldwide. Hydrogen, which is a slight and specific cytotoxic oxygen radical scavenger, has been…
Traumatic brain injuries (TBIs) are a leading cause of death and disability. Sports-related TBIs are estimated to be more than several million per year. The pathophysiology of TBIs involves high levels of inflammation, oxidative stress, dysregulation of ion homeostasis, mitochondrial…
Objective：Traumatic brain injury (TBI) is one of the most serious public health problems in the world. Hydrogen (H2), a flammable, colorless, and odorless gas, has been observed to have preventive and therapeutic effects on brain trauma and other neurological disorders,…
Traumatic brain injury (TBI) is a major cause of mortality and disability worldwide. To date, therapies to treat any forms of TBI are still limited. Recent studies have demonstrated the potential neuroprotective effects of molecular hydrogen on TBI. Although it…
Background: Sport-related mild traumatic brain injury (TBI) is a serious trauma that could impair brain function of an injured athlete. Treatment solutions for mild TBI typically concentrate on complete rest, while non-traditional therapeutic options remain largely ineffective. Molecular hydrogen (H…
0 Comments